Part:BBa_K2406083:Design

VCre Recombinase Inducible Generator

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1056
  Illegal BamHI site found at 465
  Illegal XhoI site found at 1231
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 281
  Illegal NgoMIV site found at 345
  Illegal NgoMIV site found at 712
  Illegal NgoMIV site found at 829
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 253
  

Design Notes

The VCre we initially obtained contained an illegal site in the coding sequence. We changed the codon usage of this part to make it RFC10 compatible.

Source

This part was designed from phage.